Issues:
Sections:
Issue 46
CHEMISTRY AND BIOLOGY
UDK: 577.3
INVESTIGATION OF KINETIC CHARACTERISTICS OF THE LACTATE DEHYDROGENASE IN THE GROUND SQUIRIEL GASTROCNEMIUS MUSCLE DURING HIBERNATION AND IN THE DYNAMICS OF INDUCED WARMING
KHALILOV Rustam Abdurazakovich,
DZHAFAROVA Albina Mekhyadinovna,
MEYLANOV Izzet Sirazhudinovich
Concentration dependence of lactate dehydrogenase activity in the ground squiriel gastrocnemius muscle at hibernation and in the dynamics of induced warming was investigated. Hibernation of the ground squiriel is accompanied by decrease in activity of the lactate dehydrogenase, the maximum rate doesn't change, and the Michaelis constant and the constant of substrate inhibition increase. The induced warming of a sleeping squiriel resulted in opposite effects - temperature- dependent increase of the lactate dehydrogenase activity, the maximum rate doesn't change, and the Michaelis constant and the constant of substrate inhibition decrease.
Keywords: лактатдегидрогеназа; икроножная мышца; суслики; зимняя спячка; индуцированное согревание; константа Михаэлиса; максимальная скорость; константа субстратного ингибирования; lactate dehydrogenase; ground squiriel; gastrocnemius muscle; hibernation; induced warming; maximal rate; Michaelis constant; constant of substrate inhibition.
Pages: 40 - 44
Date: 10.04.2012
Bibliography:
- Hochachka P., Somero G. Biochemical adaptation. New-York: Oxford University Press, 2002.
- Fields P.A and Houseman D.E. Decreases in activation energy and substrate affinity in cold-adapted A4-Lactate dehydrogenas: evidence from the Antarctic Notothenioid fish Chaenocephalus Aceratus // Mol. Biol. And Evol. 2004. Vol. 21. P. 2246–2255.
- Somero G.H. Adaptation of enzymes to temperature: searching for basic “strategies”// Comp. Biochem. Physiol. Part B. 2004. Vol. 139. P. 321–333.
- Activity, stability and structural studies of lactate dehydrogenases adapted to extreme thermal environments / N. Coquelle, E. Fioravanti, M. Weik, F. Velliuex, D. Madern // J. Mol. Biol. 2007. Vol. 374. P. 547–562.
- Халилов Р.А., Джафарова А.М., Мейланов И.С. Исследование кинетических характеристик лактатдегидрогеназы икроножной мышцы крыс при гипотермии // Изв. вузов. Сев.-Кав. регион. Естеств. науки. 2011. № 1. С. 75–78.
- Калабухов Н.И. Зимняя спячка млекопитающих. М.: Наука, 1985. 260 с.
- Lowry D.H., Rosembrough H.J., Farr A.L. Protein measurement with the Folinphenol reagent // J. Biol. Chem. 1951. Vol. 193. P. 265–275.
- Effects of temperature acclimation on lactate dehydrogenase of cod (Gadus morhua): genetic, kinetic and thermodynamic aspects / M. Zakhartsev, T. Johansen, H. Dörther, R. Blust // J. Experimental Biology. 2004. Vol. 207. P. 95–112.
- Storey K.B., Storey J.M. Metabolic rate depression in animals: transcriptional and translational controls // Biol. Rev. Camb. Philos. Soc. 2004. Vol. 19. P. 207–233.