Issues:
Sections:
Issue 94
GENERAL BIOLOGY
UDK: 633.81:57.085.2
OPTIMIZATION OF OBTAINING STEVIA REBAUDIANA BERTONI SUSPENSION CULTURE IN VITRO
GREVTSOVA Svetlana A.,
TSUGKIEV Boris G.,
GAGIEVA Larisa Ch.,
HOZIEV Alan M.,
KABISOV Ruslan G.,
CHERCHESOVA Susanna K.
The possibility of using a suspension culture of the herbaceous plant Stevia rebaudiana Bertoni to obtain a biologically active substance (stevioside) has been investigated. Stevia leaves are a source of diterpene glycosides, such as stevioside and rebaudiosides which are 100-300 times sweeter than sucrose and are used for functional nutrition in patients with diabetes. The results of the experiment make it possible to optimize the conditions for growing the suspension culture of Stevia rebaudiana Bertoni for further research and industrial production: composition of the nutrient medium, initial concentration of cells and duration of passage. Established have been the duration of cell cultivation and the optimal amount of the mineral component Se in the Murashige - Skoog nutrient medium in the amount of 0,5 mg for obtaining a loose callus and suspension culture of Stevia rebaudiana Bertoni. It has been found that the best growth of the culture is ensured by adding the suspension with initial density for subcultivation of 0,8?10<sup>5</sup> cells/m. The cytophysiological characteristics of the cell population are presented. During deep cultivation, density of the suspension increased by 28 times. It has been shown that the duration of biomass accumulation of a suspension culture is shorter than that for callus tissue. Throughout all the phases of the growth cycle, cell viability reaches 79%. The data obtained can be used in the field of microbiology and bioengineering to create modified scaffolds for tissue-engineered structures.
Pages: 27 - 34
Date: 15.11.2024