Issues:
Sections:
Issue 96
GENERAL BIOLOGY
UDK: 663.125/663.252.4
MORPHOLOGICAL PROPERTIES OF THE YEAST SACCHAROMYCES CEREVISIAE IN THE PROCESS OF ADAPTATION TO EXTREME CONDITIONS
The morphological properties of the Saccharomyces cerevisiae Y-503 and DAW-3a yeasts have been studied in the process of adaptation to extreme values of temperature, NaCl and pH in the composition of a solid yeast culture medium. At 30°C, the cell shape of the S. cerevisiae Y-503 strain is mostly oval, with an increase in temperature to 37° C - more rounded; the largest number of elongated forms are found in acidic media and their absence in an alkaline medium. The characteristic feature of the DAW-3a strain in all cultivation modes is the rounded shape of the cells. It is noted that under optimal conditions, cell size, especially Y-503, increases. The minimum cell sizes in all variants are found in acidic media at pH 3.0; an increase in the concentration of NaCl in the medium also leads to a decrease in size. At 30°C and pH 3.0; 4.5; 7.0; 9.0; 11.0, the sizes of giant colonies of S. cerevisiae Y-503 significantly exceed those of DAW-3a; pH 4.5 is optimal for yeast growth. At elevated temperatures of 37°C and 5, 10, 15, 20% NaCl in the medium, the strains react identically to stress, while variants with neutral and alkaline pH are more comfortable for yeast growth. The study of the tolerance of S. cerevisiae yeast Y-503 and DAW-3a to extreme conditions is of interest for possible use of stress-resistant strains in various biotechnologies.
Pages: 33 - 39
Date: 30.03.2025