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ISSN  1684-792X

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Issue 53

CHEMISTRY AND BIOLOGY

UDK: 577.22, 577.23, 577.18

MITOCHONDRIA: TEST-SYSTEM FOR EVALUATION OF TOXICITY OF AMPHIPHILIC COMPOUNDS

ALIVERDIEVA Dinara Alievna


Mitochondria play a central role in energy metabolism of eukaryotes, performing the functions of cellular respiration and providing the most of the ATP synthesis - the main source of energy in the living cell . It is shown that the mitochondria can be used as a noncontact and noninvasive biosensor for evaluation of toxic effects of the amphiphilic chemical compounds. Quantitative and qualitative parameters of effects on mitochondria of antimicrobial peptides - mastoparan, melittin and alamethicin (inducing permeability of biological membranes) defining the molecular mechanism of their action were shown. The advantages and disadvantages of mitochondrial biosensor as compared to traditional artificial systems (BLM, liposomes) were discussed. The proposed approach may be effective for the evaluation of toxic effects of amphiphilic mitochondria targeted compounds on mitochondria in a comparative routine testing of perspective drugs.

Keywords: митохондрии; биосенсор; амфифильные соединения; токсичность; тест-система; mitochondria; biosensor; amphiphilic compounds; toxicity; test-system.

Pages: 53 - 59

Date: 12.03.2014

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